Comparison of Single and Combined Use of Ergothioneine, Ferulic Acid, and Glutathione as Antioxidants for the Prevention of Ultraviolet B Radiation-Induced Photoaging Damage in Human Skin Fibroblasts

Ultraviolet B (UVB) irradiation can cause human skin damage or aging and wrinkle formation through photochemical reactions. Antioxidative substances may ameliorate UV damage. In this study, the anti-photoaging activity of three antioxidants—ergothioneine, ferulic acid, glutathione—was investigated after UVB Hs68 fibroblast cells. The cells treated with these antioxidants appeared similar to unirradiated control decreased cell viability by 26% compared that However, addition either single combined enhanced irradiation. These inhibit production reactive oxygen species (ROS) induced Ergothioneine showed a greater inhibitory effect on matrix metalloproteinase-1 (MMP-1) performance than other two antioxidants. IL-1 alpha was not detected in exposure radiation dose 150 mJ/cm2. better restoration type 1 procollagen acid glutathione. Based results, expected restore Ι production. summary, results demonstrate tested protect against UVB-induced use ergothioneine, glutathione has potential for development as materials cosmetic applications.